Effects of ethanol extract of Cenostigma macrophyllum Tul. (caneleiro) on reproductive parameters of female rats

Denise Barbosa Santos, Jamylla Mirck Guerra de Olveira, Martins Neto Bueno, Paulo Alex Bezerra Sales, Charllyton Luis Sena Costa, Mariana Helena Chaves, Maria do Carmo Carvalho e Martins, Amilton Paulo Raposo Costa

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Introduction: Cenostigma macrophyllum Tul., known as Caneleiro presents anti-inflammatory, antinociceptive, antiulcerogenic activities.
Objective: to evaluate the effects of the ethanol extract from C. macrophyllum Tul. leaves on the reproductive parameters of female Wistar rats.
Methods: for estrogenic activity assay were used 32 ovariectomized female rats (n= 8) treated for four days: I- NaCl 0.9 %, 10 mL/kg of body weight orally + corn oil 1 mL/kg intramuscular (IM); II- NaCl 0.9 % orally + estradiol 10 µg/kg bw IM; III- extract 500 mg/kg orally + corn oil IM and IV- extract orally + estradiol IM. The uteri were weighted. The estrous cycle evaluation was made using 12 female rats examined daily by vaginal smear, before, after and during the treatment (16 days) with extract (500 mg/kg). The each estrous cycle phase duration and the interval between the cycles were measured. For reproductive toxicity study were used 16 female rats (n= 8) that were treated orally during all gestational period with: NaCl 0.9 % 10 mL/kg and extract 500 mg/kg. The rats were anesthetized and laparotomized for uterus and fetus evaluation. Furthermore, the heart, liver and kidneys were collected and submitted to histopathological evaluation.
Results: the uterine weight did not differ between the groups. There was a decrease in the estrous number and an increase in the estrous cycle duration in the treatment and pos treatment groups. The extract did not cause toxicity in pregnant rats nor signs of alterations in the newborns. The histopathological analysis did not reveal significant alterations in the organs.
Conclusion: the extract interferes in the estrous cycle but does not present toxicity over gestation and nor alters macroscopically or microscopically the analyzed organs structures.